Title Optimalization of the CRISPR/CAS9 knock-in technology and application in salmon and trout
Contact Type (FP7)
Activity Area (FP6)
Regional Area (Interreg)
Specific Programme (FP7)
Funding source National-European
Coordinator Rolf Edvardsen
Coordinator email NA
IMR - Institute of Marine Research (Norway)
INRA - French National Institute for Agricultural Research (France),
MPI - Max Planck Institute for Developmental Biology (Germany),
Start year 2017
End year 2020
Funding (€) € 1,755,000
Summary The primary aim of the project is to optimize the CRISPR/Cas9 knock-in technology in zebrafish and establish the protocol for salmon and trout.
The secondary aim is to utilize these tools to explain mechanisms behind some key biological features in fish such as time of maturity (Ayllon et al. 2015), pigmentation (Irion et al. 2016), immune functions (Kjærner-Semb et al. 2016), sex determination (Herpin and Schartl 2011, Herpin and Schartl 2015), reproduction (Taranger et al.2010)and egg quality(Bobe and Labbe 2010).
In order to achievethis we will build on exsiting expertice in the consortiumon knock-in in zebrafish and established protocols formicroinjection and CRISPR/Cas9 knock-out in the salmonides developed by the consortiumpartners.The main objectivestoaccomplish theaim will be:
1. Improvement ofthe knock-inprotocol in zebrafish(primarily) and Medaka bytesting different designs of donor DNAconstructs and Cas9 proteins;
2. Implementing the best practices for knock-in from zebrafishand Medaka to salmon and trout;
3. Utlizing knock-in of putative important genetic variants insalmon and trout. Our hypothesis is that the enhanced CRISPRmethodology will be transferablefrom the model species tofarmedspecies,enabling functional studiesof key life history traits,which will ultimately lead to genetically improved aquaculture fish. Results fromthis will generate alarger knowledge base for aquaculture and also explore whether some of thesetraits may be transferable tofarmingof salmonids.
Not associated to marine areas
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